… activate the NO dioxygenase function of Escherichia coli flavohemoglobin. Steady-state and
… E and F-helices and switches an electron transfer gate formed by LysF7, GlnE7, and water. …

… of the Escherichia coli flavoHb-NOD [8,9]. However, in the resting state, the Fe(III)-flavoHb
binds … and the bound ‘Coulomb blockade’ water molecule poised between the FAD C8M and …

… -detoxifying NO metabolic activity with the flavoHb in Escherichia coli (Gardner et al. 1998a;
… modeled on the E. coli ferric flavoHb with a bridging Coulomb blockade water molecule (W) …

… It is soluble in water (approximately 1.6 mM at 37 C and 1.94 … to the NO dioxygenase
activities of flavohaemoglobin 12 . … Thus, for example, the Escherichia coli flavohaemoglobin (…

… Under these conditions, growth can only resume once nitric oxide (NO) is detoxified by the
flavohemoglobin Hmp. Paradoxically, S. epidermidis produces endogenous NO through its …

… Hence, the Fhb, also known as NO-dioxygenase, can … in an ice bath and a boiling water bath
three times for 10 min. … washed twice with 1 mL of distilled water, and re-suspended in 1 mL …

… (FlavoHbs) function as nitric oxide dioxygenases, oxidizing … known as Alcaligenes eutrophus),
Escherichia coli [[6]] and … interacts with both the bridging water group and the propionate …

… fHbs exhibit a nitric oxide dioxygenase (NOD) activity which … of electron transfer in
flavohemoglobins, specifically disruption of the … groups through adjacent structural water …

… Hmp is the flavohemoglobin of Escherichia coli, and here we … (resistivity of 18.2 MΩ ×
cm) in a ratio of 25 g LB mix/L water, … of Escherichia coli nitric-oxide dioxygenase (flavohemoglobin…

Mycobacterium tuberculosis (Mtb) produces an unconventional flavohemoglobin (MtbFHb)
that carries a FAD-binding site similar to D-lactate dehydrogenases (D-LDH) and oxidizes D-…